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Neuromuscular junctions in normal skeletal muscle. Esterase preparation (A) darkly highlights neuromuscular junctions (NMJs) along a small intramuscular nerve twig. Original magnification: A. Nonspecific Esterase Preparation, 100x. A relatively young patient presented with hypotonia. Course features were noted on physical exam; however, multiple studies revealed no etiology for the patient’s clinical picture. A muscle biopsy was pursued to evaluate for myopathy. Using Figure 1, what structures (black arrows) are highlighted by the esterase stain shown below? Answer: Neuromuscular Junctions Nonspecific esterase preparation is one of the principal enzymatic histochemical stains used to evaluate neurogenic pathology in skeletal muscle...