April 14, 2017
Necrotizing vasculitis as shown using fluorescence with DAPI-FITC-Texas Red excitation on an H&E stained section. Subtle and not so subtle necrotizing areas are seen easily using this fluorescence technique. The section shows necrotizing vasculitis in an 87-year-old male who presented with hematuria, proteinuria and a creatinine of 2.5 mg/dl. Following the biopsy, ANCA serologies were ordered with cANCA positivity.